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Image Search Results
Journal: Neural Regeneration Research
Article Title: Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma
doi: 10.4103/1673-5374.353848
Figure Lengend Snippet: Damage to the ONL and INL worsens on the seventh day after blunt ocular trauma. (A) Representative images of ChAT staining (green; cholinergic cells). Compared with the number of ChAT-positive cells in the control group, the number of ChAT-positive cells began to decline on the seventh day after injury. (B) The proportion of ChAT-positive cells was recorded after blunt ocular trauma and normalized to data from the control group. (C) Representative images of calretinin staining (green; amacrine cells). Compared with the number of calretinin-positive cells in the control group, the number of calretinin-positive cells also began to decline on the seventh day after injury. (D) The proportion of calretinin-positive cells was recorded after blunt trauma and normalized to data from the control group. (E) Representative images of calbindin-D staining (green; horizontal cells). Compared with the number of calbindin-D-positive cells in the control group, the number of calbindin-D-positive cells did not change. (F) The proportion of calbindin-D-positive cells was recorded after blunt trauma and normalized to data from the control group. (G) Representative images of PKCα (PKC, red; bipolar cells) and synaptophysin (Syn, green; photoreceptor ribbon synapses) staining. Compared with the number of PKC-positive cells in the control group, the number of PKC-positive cells significantly decreased on the first day after injury. Compared with the number of PKCα and synaptophysin double-positive cells in the control group, the number of PKCα and synaptophysin double-positive cells was reduced on the seventh day after injury. (H) The proportion of PKC-positive cells was recorded after blunt ocular trauma and normalized to data from the control group. (I) The percentage overlap of PKCα and synaptophysin double-positive cells was recorded after blunt ocular trauma. Scale bar: 100 μm. Data are shown as the mean ± SEM ( n = 5 per group). * P < 0.05, vs . control group (Student’s t -test). ChAT: Choline acetyltransferase; PKC: protein kinase C.
Article Snippet: Sections were then incubated at 4°C overnight with one or more of the following primary antibodies [all diluted 1:100 by a fresh blocking buffer (0.01 M PBS containing 0.3% Triton X-100 and 3% bovine serum albumin)]:
Techniques: Staining, Control